自拟通痹汤对Ⅱ型胶原性关节炎关节软骨的作用

Effect of self-prescribed Tongbi decoction on the articular cartilage following typeⅡcollagen arthritis

背景:自拟通痹汤是喻建平教授临床观察多年的临床经验方,适用于类风湿关节炎湿热痹阻证。目的:验证自拟通痹汤对Ⅱ型胶原性关节炎大鼠的足肿胀的抑制作用,以及对自身抗体IgG、T细胞亚群、细胞因子白细胞介素1β和肿瘤坏死因子α的调节作用及对踝关节局部病理损伤的治疗作用。方法:Wistar大鼠分为6组。除正常组外,以Ⅱ型胶原建立大鼠Ⅱ型胶原性关节炎动物模型。模型组:给予给药大剂量组等体积的蒸馏水;对照组:给予泼尼松5.0mg/kg;自拟通痹汤大、中、小剂量组,剂量分别为11.6,5.8和2.9g/kg。正常对照组大鼠右后足趾皮下注射生理盐水0.1mL。1周后以相同剂量于每只大鼠尾部加强注射1次。造模2周后开始给药,均为灌胃给药,1次/d,连续2周。结果与结论:结果表明,自拟通痹汤对Ⅱ型胶原诱导的大鼠关节炎关节肿胀有明显的抑制作用;对大鼠血清IgG水平升高有明显的抑制作用;对大鼠胸腺CD4+和CD8+T淋巴细胞的异常有明显的免疫调节作用;对大鼠血清肿瘤坏死因子α有明显的抑制作用。自拟通痹汤各剂量组大鼠滑膜水肿、炎性细胞浸润、滑膜增生、血管翳生成、软骨破坏等病理损害均得到改善。

BACKGROUND：The self-prescribed Tongbi decoction is an empirical formula that has been observed in clinic for many years by Prof.Yu Jian-ping,and it is applied to treat dampness-heat blockage syndrome of rheumatoid arthritis.OBJECTIVE：To verify the inhibitory effect of self-prescribed Tongbi decoction on the hind paw swelling in adjuvant-induced arthritis（AIA） rats,and its regulative effect on autoantibody IgG,T cell subgroups,interleukin 1β and tumor necrosis factor α,as well as its therapeutic role on ankle pathological injuries.METHODS：Wistar rats were divided into six groups.An AIA rat model with collagen Ⅱ was established in all groups except for the normal control group.The rats of the model group were injected an equal volume of distilled water as the high-dose administration group;rats of the control group were given 5.0 mg/kg prednisone;rats in the high-dose,moderate-dose and low-dose administration groups were treated with 11.6,5.8 and 2.9 g/kg self-prescribed Tongbi decoction,respectively.The rats in the normal control group were injected 0.1 mL normal saline into the rear toe of rats.After 1 week,all rats received a booster injection with the same dose on the tails.After modeled for 2 weeks,the rats were given intragastric administration,once a day for 2 weeks.RESULTS AND CONCLUSION：The self-prescribed Tongbi decoction has a significant inhibitory effect on the hind paw swelling in collagen-induced arthritis rats,and a significant inhibitory effect on the increase of IgG serum concentration,a significant immunomodulatory effect on the abnormal CD4,CD8 and T lymphocytes as well as a significant inhibitory effect on tumor necrosis factor α.The synovial membrane edema,inflammatory cell infiltration,synovial proliferation,pannus generating and cartilage destruction achieved an improvement in different doses of self-prescribed Tongbi decoction groups.