线粒体酶活性及线粒体肿胀与不同运动强度的关系

Effects of different exercise intensities on the activity of mitochondrial enzymes and mitochondrial swelling

背景:关于不同运动强度对机体的影响早有研究,并且取得了不少有价值的成果。但是对于不同运动强度对线粒体Na+,K+-ATP酶、Ca2+-ATP酶活性以及线粒体肿胀程度影响的研究较少。目的:观察不同运动强度对大鼠骨骼肌线粒体Na+,K+-ATP酶和Ca2+-ATP酶以及线粒体肿胀的影响。方法:将24只SD大鼠随机分为正常对照组,中等强度运动组和高强度运动组。正常对照组大鼠正常笼内生活,不运动。另2组游泳训练方式,分别建立中强度和高强度运动模型,进行相应的运动锻炼,训练4周。结果与结论:中强度运动组线粒体Na+,K+-ATP酶、Ca2+-ATP酶活性与正常对照组相比均增高(P<0.05),线粒体肿胀程度降低(P<0.05);而高强度运动组线粒体Na+,K+-ATP酶、Ca2+-ATP酶活性与正常对照组相比均降低(P<0.05),线粒体肿胀程度增大(P<0.05)。实验结果表明中强度运动可以保护线粒体Na+,K+-ATP酶、Ca2+-ATP酶的活性,提高线粒体功能;而高强度运动则降低了线粒体的功能。

BACKGROUND：There are many studies on the effects of different exercise intensities on the body,but studies about the effects of different exercise intensities on the activity of mitochondrial Na＋,K＋-adenosine triphosphatase（ATPase）,Ca2＋-ATPase and mitochondrial swelling are few.OBJECTIVE：To investigate the effects of different exercise intensities on the activity of mitochondrial Na＋,K＋-ATPase and Ca2＋-ATPase,as well as mitochondrial swelling.METHODS：Twenty-four Sprague Dawley rats were randomly separated into three groups,namely,control,mid-intensity,high-intensity exercise groups.The control rats did not undergo swimming training,while the other two groups were given 4-week swimming exercises of different intensities.RESULTS AND CONCLUSION：Compared with the control group,the Na＋,K＋-ATPase and Ca2＋-ATPase activities increased（P0.05） and the extent of mitochondrial swelling decreased（P0.05） in the mid-intensity exercise group,but opposite in the high-intensity exercise group.These findings indicate that mid-intensity exercise can protect the activity of mitochondrial Na＋,K＋-ATPase and Ca 2＋-ATPase and increase the function of mitochondria,but high-intensity exercise can decrease the function of mitochondria.