苯妥英钠的抑菌活性

The antibacterial activity of phenytoin

背景:苯妥英钠在创面愈合过程中的抑菌作用仍不明确。目的:通过体外及动物实验观察苯妥英钠在创面愈合过程中是否具有抑菌作用。方法:①体外实验:采用水解酪蛋白肉汤稀释法检测苯妥英钠的最低抑菌浓度、琼脂扩散法观察1024mg/L的苯妥英钠的体外抑菌活性。②动物实验:将30只SD大鼠随机等分为3组,在大鼠背侧脊柱旁设计一2cm×3cm矩形创面,分别用10,20mg/cm2苯妥英钠糊剂或单纯凡士林纱布进行处理,创面均外加凡士林油纱及干纱包扎固定。隔日换药,于第4,8,12,16天进行创面表面细菌定量培养。结果与结论:①水解酪蛋白肉汤稀释法结果:应用质控菌株、临床菌株进行试验,不同苯妥英钠浓度的试管中均呈现浑浊状态。②琼脂扩散法结果:应用质控菌株进行试验,未发现抑菌圈的形成。③实验动物创面细菌定量培养结果:应用10,20mg/cm2苯妥英钠糊剂处理的创面细菌定量略低于单纯凡士林纱布处理的创面,但各组之间差异均无显著性意义(P>0.05)。表明苯妥英钠尚无明确的体外及体内抑菌活性,对创面细菌的清除无明显作用。

BACKGROUND：The antibacterial activity of phenytoin in wound healing process is still not clear.OBJECTIVE：To observe the antibacterial activity of phenytoin in the wound healing process through in vitro and animal experiment.METHODS：①In vitro experiment：Casein hydrolyzate broth dilution was used to observe the minimum inhibitory concentration,and agar diffusion method was used to observe in vitro antibacterial activity of 1.024mg/L phenytoin.②Animal experiments：Thirty Sprague Dawley rats were divided into three equal groups;a 2cm×3cm rectangular wound was created in rat dorsal paraspinal,and then treated with 10 or 20mg/cm2 phenytoin paste or pure Vaseline gauze,the wound was bandaged and fixed with Vaseline yarn and dry gauze.The dressing was changed every day,and the wound surface bacteria were quantitative cultured at 4,8,12 and 16 days.RESULTS AND CONCLUSION：①Casein broth dilution method results：The quality control strains and clinical isolates test were performed,the test tubes of different phenytoin concentration showed a turbid state.②Agar diffusion method results：The quality control strains test was performed,and no inhibition zone formation was observed.③Experimental animal wound bacteria quantitative culture results：The bacteria quantization in the group treated with 10 or 20mg/cm2 phenytoin paste was lower than that in the group treated with pure Vaseline gauze,but there was no significant difference between groups（P0.05）.The results show that the in vitro or in vivo antibacterial activity of phenytoin is not clear,and it has no significant effect on the wound bacterial clearance.