湖北籍汉族强直性脊柱炎患者白细胞介素1受体拮抗剂基因的多态性

Polymorphisms of interleukin-1 receptor antagonist gene in ankylosing spondylitis patients who are Han nationality from Hubei Province

背景:白细胞介素1是强直性脊柱炎易感基因之一,其多态性与疾病易感性相关,不同人群的研究结果不尽相同。目的:分析中国湖北汉族人群中白细胞介素1受体拮抗剂基因多态性与强直性脊柱炎的相关性。方法:自140名强直性脊柱炎患者及130名健康对照者外周血中提取DNA,采用PCR方法检测白细胞介素1受体拮抗剂基因第二内含子VNTR复等位基因多态性。所有受试者均为3代或3代以上居住于湖北的汉族人。结果与结论:强直性脊柱炎患者中白细胞介素1受体拮抗剂基因*2等位基因频率显著高于健康对照者(P<0.05)。提示强直性脊柱炎与白细胞介素1受体拮抗剂基因*2等位基因相关联。

BACKGROUND：Interleukin 1（IL-1） gene cluster contains a major susceptibility locus for ankylosing spondylitis.Its polymorphisms are correlated with the susceptibility of ankylosing spondylitis.The study results are often different in different population.OBJECTIVE：To analyze the correlation between polymorphism of IL-1 receptor antagonist（IL-1RN） gene and patients with ankylosing spondylitis.The patients are the population of Han nationality of Hubei Province in China.METHODS：DNA samples were collected from 140 patients with ankylosing spondylitis and 130 healthy as controls.The IL-1RN VNTR polymorphisms were determined by PCR method.All patients are of Han nationality and lived in Hubei Province for three generations at least.RESULTS AND CONCLUSION：Compared with the healthy controls,IL-1RN VNTR＊2 allelic frequency in patients with ankylosing spondylitis was significant higher（P0.05）.