人肺癌组织中nm23-H_1基因突变研究

Mutational analysis of nm 23-H_1 gene in human lung cancer by polymerase chain reaction single strand conformation polymorphism

目的 探讨癌转移抑制基因nm2 3 H1 在人肺癌中的突变情况 ,及其与肺癌发生、发展及转移的关系。方法 采用聚合酶链反应—单链构象多态性技术 (PCR SSCP) ,对手术切除的 45例肺癌组织和 7例正常肺组织的nm2 3 H1 基因的 5个外显子进行突变分析。结果 在所检测的肺组织中 ,未发现nm2 3 H1 基因纯合缺失。SSCP分析发现一例肺癌组织nm2 3 H1 基因外显子 1单链DNA迁移率发生改变。此例患者为晚期肺鳞癌 ,伴有纵隔淋巴结转移和恶性胸水。结论 本实验所检测的nm2 3 H1 基因外显子部分的基因突变在肺癌中发生率低 ,nm2 3 H1

Objective To investigate the mutation of nm23 g en e and its correlation with development, progression and metastasis of human lung cancer. Methods Polymerase chain reaction single strand con fo rmation polymorphism (PCR SSCP) was employed for mutational analysis of the fiv e exons of nm23 H1 gene in 45 surgically resected lung cancer and 7 normal pul monary tissues. WTHZ Results In the examined DNA samples of pulmonar y tissues, no homozygous loss of allele of nm23 H1 gene was observed. SSCP ana lysis revealed altered mobility of single stranded DNA of nm23 H1 exon 1 in one case of stage ⅢB squamous cell pulmonary carcinoma with mediastinal lymphnod e metastasis and malignant pleural effusion. Conclusion The frequency of nm23 H 1 gene mutation in its coding region is rare in lung cancer. Mutation of nm23 H 1 gene might play a role in the progression and metastasis of lung cancer.