摘要
将520只7日龄海兰灰蛋鸡雏随机分成4组,分别设为3个剂量组(0.5%组、1.0%组和1.5%组)和一个空白对照组,给药组分别于14日龄常规剂量滴鼻点眼免疫法必妥(鸡传染性法氏囊病中等毒力活疫苗),28日龄2倍剂量饮水法必妥。通过测定法氏囊病抗体水平、脾指数、法氏囊指数、外周血液酸性-醋酸萘酯酶(ANAE)阳性淋巴细胞值和外周血白细胞总数等指标,研究芪芍增免散对鸡法氏囊病疫苗免疫增强的影响。结果显示:芪芍增免散能够明显延长法氏囊病抗体持续时间,提高脾指数、法氏囊指数、外周血液酸性-醋酸萘酯酶(ANAE)阳性淋巴细胞值和外周血白细胞总数。推荐芪芍增免散1.0%组为临床推荐剂量。
520 7-day-old Hy gray eggs chickens were randomly divided into four groups inculding three experiment groups （0.5% groups, 1.0% groups, 1.5%groups） and a control group. The chickens were immunized with Fabitux （IBD moderate virulence live vaccine） by the method of intranasal immunization and eye-drop vaccination at 14-day-old and were vaccinated through water again at 28-day-old. The effect of Qishaozengrniansan on the immunological enhancement of IBD vaccine were determined by detection of the antibody levels against IBD, spleen index, acidic - naphthyl acetate esterase （ANAE） in peripheral blood lymphocytes and the leukocyte in peripheral blood. The results showed that Qishaozengmiansan could significantly prolong the bursal antibody persistence time, increase the spleen index, bursal index, ANAE＇s value and the leukocyte.count. So1% Qishaozengmiansan was recommended for clinical application.
出处
《广东畜牧兽医科技》
2012年第6期38-41,50,共5页
Guangdong Journal of Animal and Veterinary Science
