猪囊尾蚴抗原DNA疫苗诱导的免疫保护效应

Protective immunity induced by DNA vaccine of Cysticercus cellulosae antigen

目的 :观察猪囊尾蚴保护性抗原 c C1DNA疫苗诱导的小鼠免疫应答 ,及对仔猪的免疫保护作用。方法 :将全长 c C1c DNA插入真核表达质粒载体 pc DNA3,构建了重组质粒 p3- c C1,肌注小鼠 ,EL ISA检测小鼠血清抗 c C1抗体 Ig G及 Ig G2 a水平 ,并进行仔猪的免疫保护实验。结果 :免疫小鼠第 3周出现特异性 Ig G应答 ,第 8周 Ig G水平达到最高 ;小鼠血清的 Ig G2 a检测显示 ,第 2周 Ig G2 a应答即为阳性 ,且长时间保持较高应答水平。用该疫苗免疫仔猪使其获得了 73%的保护率。结论 :猪囊尾蚴保护性抗原 c C1DNA疫苗能有效诱导仔猪的免疫保护效应。

Objective: To investigate the immune response induced by Cysticercus cellulosae protective antigen cC1 DNA vaccine in mice and the protective immunity induced by immunized newborn pigs. Methods: Recombined plasmid p3 cC1 was constructed by inserting full length cC1 cDNA into an eukaryotic expression vector pcDNA3. Mice were injected intramuscularly with the recombined construct. Anti cC1 antibody and IgG 2a in serum were screened by ELISA. Then the protective immunization in pigs was done. Results: The immune response of specific antibody was induced during the 3r week. The highest level was during the 8th week. IgG 2a response was detected during the 2nd week. The higher duration of IgG 2a response induced by DNA vaccine was longlived. The protective rate induced in immunized newborn pigs was 73%. Conclusion: The cC1 DNA vaccine can effectively induce protective immunity in newborn pigs.