新城疫病毒HN基因真核表达质粒的构建及其抗肿瘤作用的初步研究

Construction of Newcastle disease virus hemagglutinin-neuraminidase (HN) gene and its antitumor immune response effects

目的 :构建新城疫病毒 (NDV )血凝素神经氨酸酶 (HN)基因真核表达质粒 ,并初步探索其在抗肿瘤治疗中的机制及应用价值。方法 :用 RT- PCR从 NDV中克隆 HN c DNA,并构建 HN的真核表达载体 ,转染 COS- 7细胞表达。注射至小鼠B16黑素瘤模型 ,检测其抗肿瘤作用。结果 :成功地克隆了 NDV HN c DNA,所构建的 pc DNA3- HN有良好的表达 ,动物实验显示 ,pc DNA3- HN有增强荷瘤小鼠 CTL ,NK杀伤活性的作用。结论 :NDV HN的真核表达质粒具有增强荷瘤小鼠抗肿瘤CTL ,NK细胞反应的作用。

Objective: To construct an eukaryotic expression plasmid containing gene coding for the hemagglutinin neuraminidase(HN) of Newcastle disease virus (NDV), and to study its mechanism and value in antitumor therapy. Methods: The HN cDNA was abtained from NDV with RT PCR and an eukaryotic expression vector of HN gene ( pcDNA3 HN ) was constructed. The antitumor effect was evaluated after injecting pcDNA3 HN into mice bearing B16 melanoma. Results: The HN cDNA of NDV was successfully cloned and pcDNA3 HN had a good expression in COS 7 cells. Animal experiments suggested that the pcDNA3 HN could significantly increase CTL and NK activity of tumor bearing mice. Conclusion:The eukaryotic expression plasmid containing the gene coding for the (HN) has the function of increasing CTL and NK activity of tumor bearing mice.