龙血竭特征成分对照指纹图谱研究

HPLC characteristic fingerprints of Resina Draconis compared with reference substances

目的:建立以5个特征成分为对照的龙血竭HPLC指纹图谱分析方法。方法:采用Phenomenex lura C18(4.6mm×250mm,5μm)柱,以甲醇-乙腈-0.2%磷酸溶液梯度洗脱,流速1.0mL/min,柱温35℃,检测波长306nm。结果:不同厂家的12批龙血竭的指纹图谱相似度在0.95以上,确定了12个共有峰,并通过对照指认了白藜芦醇、紫檀茋、龙血素A、龙血素B、7,4,-二羟基黄酮共5个特征性化学成分。结论:建立的分析方法能同步检测出龙血竭中茋类、二氢查耳酮类及黄酮类的特征性成分,为更全面评价其质量提供依据。

Objective： To establish a fingerprint compared with characteristic ingredients of Resina Draconis by RP-HPLC.Methods： The samples were separated on a Phenomenex luna C18 column with gradient elution of methanol-acetonitrile-0.2% phosphoric acid（v/v） at a flow rate of 1.0mL/min and detected at 306nm.Results： The similarity of Resina Draconis fingerprints from different company was better,the total 12 characteristic peaks were selected,and five major characteristic ingredients in Resina Draconis can be detected simultaneously,which named resveratrol,7,4＇-dihydroxyflavone,loureirin A,loureirin B and pterostilbene.Conclusion： The fingerprints can be used as a control method of stilbenes,dihydrochalcones and flavones in Resina Draconis.