水芹提取物对应激致小鼠慢性疲劳综合征的对抗作用

Therapeutic effect of extract of Oenanthe Javanica against the chronic fatigue syndrome in mice

目的:观察水芹提取物对应激致小鼠慢性疲劳综合征(CFS)的对抗作用及其相关机制。方法:采用强制冷水游泳应激法制备CFS模型,从应激角度,观察应激导致动物疲劳时,其大体行为学、下丘脑—垂体—肾上腺轴(HPA轴)功能变化、中枢神经递质的变化以及骨骼肌能量代谢指标,并进一步探讨水芹提取物治疗慢性疲劳的机制。结果:冷水应激使小鼠自主活动明显下降;HPA轴功能指标:血清促肾上腺皮质激素(ACTH)、皮质醇(CORT)明显升高,性激素睾酮(T)水平下降;中枢递质指标:5-羟色胺(5-HT)、多巴胺(DA)明显上升;外周疲劳骨骼肌能量代谢指标:超微量Ca2+-Mg2+-ATP酶活力降低、磷酸(Pi)含量明显升高,体内自由基水平丙二醛(MDA)明显升高、超氧化物歧化酶活力(SOD)明显下降。药物治疗后,小鼠自主活动能力明显增加、HPA轴与性腺轴功能恢复正常,中枢神经类递质降低,外周疲劳情况好转,体内自由基消除到正常水平。结论:水芹提取物对小鼠CFS具有明显的治疗作用。

Objective： To explore the role and the mechanism of the extract of Oenanthe Javanica（OJE） against the CFS in mice.Methods： We prepared the stressed CFS model by forcing mice swimming in cold water.From the perspective of stressed,we observed the general behavior of every mouse,function of the axis of Hypothalamus-Pituitary-Adrenal and the energy metabolism in skeletal muscle,and then made a further explore for the mechanism of the treatment of chronic fatigue syndrome.Results： The results showed that cold water stress can significantly decrease the locomotor activity of mice,the levels of ACTH,CORT in the serum of the axis of Hypothalamus-Pituitary-Adrenal significantly were increased,the level of sex hormone testosterone was decreased,the levels of 5-HT,DA of central neurotransmitters were significantly increased,the vitality of Ca2＋-Mg2＋-ATP enzyme was decreased,the level of Pi was significantly increased,the level of Radical such as MDA significantly was increased,the vitality of SOD significantly was decreased.After OJE＇s treatment,the locomotor activity of mice was significantly increased,function of the axis of Hypothalamus-Pituitary-Adrenal and Gonadal axis returned to normal,the level of central neurotransmitters was decreased,situation of peripheral fatigue was improved,the level of radical was back to normal.Conclusion： OJE can significantly therapy the chronic fatigue syndrome in mice caused by chronic stress.