迷迭香精油通过嗅觉通路改善小鼠学习记忆的研究

Improvement of learning and memory behavior in mice through olfactory pathwayusing essential oil of Rosmarinus officinalis

目的探讨迷迭香精油通过嗅觉通路改善C57BL/6小鼠学习记忆能力及其可能的机制。方法通过鼻吸入迷迭香精油的方法 ,以毁损嗅上皮模型作对照,分组进行Lashley-III水迷宫和嗅觉辨识记忆实验,并进行免疫组化,观察各组小鼠海马CA1区乙酰胆碱酯酶、谷氨酸的表达情况。结果迷迭香精油组小鼠的水迷宫实验和嗅觉辨别记忆实验潜伏期低于其他组(P<0.05),免疫组化结果显示该组小鼠海马CA1区乙酰胆碱酯酶、谷氨酸的表达增强。结论迷迭香精油能够通过嗅觉通路改善小鼠的学习记忆功能,其机制可能与嗅觉通路中神经递质的改变有关。

Objective To study the fimction ofRosmarinus officinalis essential oil （ROEO） on improving the learning and memory behavior in C57BL/6 mice through olfactory pathway and its possible mechanism. Methods The ROEO was given via inhalation and the damaged olfactory epithelium of mice was taken as the control model. The Lashley-III water maze and olfactory discrimination memory tests of mice were carried out and the expressions of AchE and Glu in hippocampus CA1 of mice were observed by immunohistochemistry in different groups. Results The latency in water maze test and olfaction discrimination memory tests of mice in ROEO group was lower than that of mice in the other groups （P〈0.05）, and the expressions of AchE and Glu in hippocampus CA1 of mice in ROEO group was significantly increased by immunohistochemistry. Conclusion ROEO could improve the learning and memory behavior of mice through olfactory pathway. The mechanisms are related to the changing of brain＇s neurotransmitters.