HPLC法测定女金糖浆中橙皮苷、黄芩苷和丹皮酚

Determination of hesperidin,baicalin and paennol in Nüjin Syrup by HPLC

目的建立女金糖浆中橙皮苷、黄芩苷和丹皮酚的HPLC分析方法。方法采用资生堂Spolar-C18色谱柱(250mm×4.6mm,5μm);流动相为[甲醇-乙腈-0.1%磷酸(12:13:75)]-乙腈,进行梯度洗脱;柱温40℃;体积流量1.0mL/min;检测波长283nm;进样量10μL。结果橙皮苷、黄芩苷和丹皮酚分别在0.07395～2.21849、0.50820～10.16400、0.15871～2.38060μg线性关系良好,回收率分别为97.0%、98.9%、99.0%,RSD值分别为0.33%、0.41%、0.14%(n=6)。结论该方法方便、快速、灵敏度高,可以为女金糖浆的质量控制提供依据。

Objective To establish an HPLC method for the determination of hesperidin, baicalin, and paeonol in Niijin Syrup. Methods The samples were separated on Shiseido Spolar-C18 column （250 mm×4.6 mm, 5μm） with [methanol-acetonitrile-0.1% phosphoric acid （12 ： 13 ; 75）]-acetonitrile as mobile phase by gradient elution. The detection wavelength was 283 nm and column temperature was 40 ℃ with injection volume of 10 μL at a flow rate of 1.0 mL/min. Results The calibration curves were linear over the range of 0.073 95--2.218 49 μg, 0.508 20--10.164 00, and 0.158 71--2.380 60 ktg （r=0.999 1） for hesperidin, baicalin and paeonol. The average recovery rates were 97.0%, 98.9%, and 99.0% with RSD at 0.33%, 0.41%, and 0.14%, respectively （n=6）. Conclusion This method is simple, rapid, and sensitive, and could provide the basis for the quality control of Nujin Syrup.