HPLC法测定心脑络通颗粒中野黄芩苷和4,5-二-O-咖啡酰奎宁酸

Determination of scutellarin and 4,5-dicaffeoylquinic acid in Xinnao LuotongGranules by HPLC

目的建立HPLC法同时测定心脑络通颗粒中野黄芩苷和4,5-二-O-咖啡酰奎宁酸的方法。方法色谱柱为岛津InterstilODS柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%三氟乙酸(25:75),体积流量1.0mL/min,检测波长335nm,柱温37℃,进样量10μL。结果 2种成分均能达到基线分离,重现好,且呈现较好的线性关系;加样回收率分别为97.1%、99.8%,RSD值分别为0.66%、0.82%。结论该方法准确、灵敏、可靠,重复性好,可用于心脑络通颗粒的快速检测。

Objective To develop an HPLC method for simultaneous determination of scutellarin and 4,5-dicaffeoylquinic acid in Xinnao Luotong Granules. Methods The samples were separated on Shimadzu Interstil ODS column （250 min×4.6 mm, 5 μm） with acetonitrile-0.1% trifluoroacetic acid （25 ： 75） as mobile phase. The detection wavelength was 335 nm and column temperature was 37 ℃ with injection volume of 10 μL at a flow rate of 0.8 mL/min. Results Two active components were well separated with good repeatability and linearity. The calibration curves were linear. The average recovery rates were 97.1% and 99.8% with RSD values at 0.66% and 0.82%, respectively. Conclusion The method is accurate, sensitive, credible, and repeatable, and could be used for the rapid detection of Xinnao Luotong Granules.