抗体人源化及人源抗体研究技术
摘要
抗体研究已有百余年的历史，但直到20世纪70年代，随着杂交瘤技术的问世，抗体的制备和生产技术才实现革命性的突破。近年来，抗体以其高靶向性成为一大类新型诊断和治疗剂，在疾病的诊断和治疗中得到了广泛的应用。目前治疗性抗体药物在全球生物技术药品的市场份额己位居第二。同时，
出处
《生物产业技术》
2012年第6期15-20,共6页
Biotechnology & Business
参考文献22
-
1Wang Y, Feng 3, Huang Y, et al. The design, construction and function of a new chimeric anti-CD20 antibody. 3 BiotechnoL 2007, 129(4):726-731.
-
2ttou S, Li B, Wang L, et al. Humanization of an anti-CD34 monoclonal antibody by cola lementarity-determining region grafting based on computer-assisted molecular modeling. J Biochem, 2008 Apr It); [Epub ahead of print].
-
3Zhang W, Feng J, Li Y, et llumanization of an anti- al. human TNF-alpha antibody by variable region resurFacing with the :id of molecular modeling. Mol lmmunol, 2005, 42(12): 1445-I451.
-
4Chang H, Qin W, Li Y, et al. A novel human scFv fragment against TNF-alpha from de novo design method. Mol Immunol, 2007, 44(15 ): 3789-3796.
-
5Lv M, Li Y. Yu M. et al. Structured to reduce the mitogenicity of anti-CD3 antibody based on computer-guided molecular design. Int J Biocbem Cell Biol. 20(17. 39(61: 1142- 1155.
-
6Qin W, Feng J, et al. A novel domain antibody rationally designed against TNF-alpha using variable region of human heavy chain antibody as scaffolds to display antagonistic peptides. Mol lmmunol, 2007, 44(9): 2355-2361.
-
7Lin Z, Cao P, Lei H. Identification of a neutralizing scFv binding to human vascular endothelial growth factor 165 (VEGFI65) using a phage display antibody library. Appl Biochem Biotechnol, 2008, 144(1): 15-26.
-
8Shibuya T, Yamashiro T, Masaike Y, et al. Identification of a human monoclonal Fab with neuta-alizing activity against ]H3N2 influenza A strain from a newly constructed human Fab library. Microbiol lmmunol, 2008, 52(3): 162-170.
-
9Rothe C, Urlinger S, L6hning C, et al, The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immtme system with a novel display method for efficient selection of high-affinity antibodies. J Mol Biol, 2008, 376(4): 1182-1200.
-
10Cesaro-Tadic S, Lagos D. Honegger A, et al. Turnover- based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. Nat Biotechnol. 2003, 21(6): 679-685.
-
1张明合,吴立清,梁俊.人源抗体的研究现状[J].安徽教育学院学报,2000,18(3):46-47.
-
2汪兴泽.人源抗体缘何须改造[J].生物学教学,2010,35(9):58-59.
-
3袁斌.金属螯合亲和层析法纯化人源抗TNF—α重链可变区蛋白[J].细胞与分子免疫学杂志,2000,16(6):499-500.
-
4信息荟萃[J].医药工程设计,2005,26(2):47-49.
-
5王臣,温文彦,张春杰.鼠源单克隆抗体人源化研究进展[J].安徽农业科学,2006,34(11):2336-2337. 被引量：4
-
6袁斌,何君,俞晓峰,王慧,刘怀田,周育森,黄策.人源抗肿瘤坏死因子-α噬菌体抗体的基因结构分析[J].生物技术通讯,2000,11(1):5-8. 被引量：1
-
7潘康成,吕道俊,何明清.正常菌群对动物免疫功能的影响研究进展[J].饲料工业,1999,20(9):24-26. 被引量：39
-
8抗体高通量体外筛选技术及规模制备的构建[J].中国科技成果,2010(5):9-10.
-
9徐俊杰,吕士杰,魏景艳.含硒抗体模拟谷胱甘肽过氧化物酶的研究进展[J].吉林医药学院学报,2010,31(4):220-223. 被引量：4
-
10寻求一种生物基因处方[J].中国高新技术企业,2004(3):90-91.
