HPLC测定不同产地灵芝中9种三萜酸

Determination of nine triterpenoid acids from Ganoderma lucidum of different producting areas by HPLC

目的:建立HPLC测定灵芝子实体中9种三萜酸的方法。方法:采用Alltima C18色谱柱(4.6 mm×150 mm,5μm),流动相为乙腈-0.04%甲酸溶液,检测波长254 nm,流速1.0 mL.min-1,柱温15℃。结果:灵芝酸C2、灵芝酸G、灵芝烯酸B、灵芝酸B、灵芝烯酸A、灵芝酸A、赤芝酸A、灵芝烯酸D、灵芝酸C1的线性范围分别为6.81～40.88,6.38～38.25,6.75～40.50,6.38～38.25,5.95～35.65,5.90～35.25,7.00～42.00,6.20～37.15,6.05～36.4 mg.L-1(r=0.999 4,0.999 2,0.999 4,0.999 2,0.999 2,0.994 5,0.999 0,0.999 2,0.998 4),加样回收率分别为102.1%,102.3%,100.6%,103.3%,104.1%,103.2%,96.42%,102.5%,101.5%,RSD为1.5%,0.96%,1.9%,1.3%,1.7%,2.5%,0.62%,2.9%,1.3%。测定了31个不同地区、不同栽培条件下灵芝样品的三萜含量。结论:该方法可行、重复性好,可定量测定灵芝中三萜酸的含量。

Objective： To establish an HPLC method for determining nine triterpenes contained in Ganoderma lucidum. Method： Chromatography conditions： Alltima C18 （4. 6 mm × 150 mm, 5 μm） was adopted as the chromatographic column, with acetonitrile-0. 04% formic acid solution as the mobile phase. The detective wavelength was set at 254 nm, and the column temperature was 15 ℃. Result： The linearities of ganoderic acid C2 , ganoderic acid G, ganoderenic acid B, ganoderic acid B, ganoderenic acid A, ganoderie acid A, lucideric acid A, ganoderenic acid D, and ganoderic acid Cl ranged between 6. 81-40. 88, 6. 38-38.25, 6.75- 40. 50, 6. 38-38.25, 5.95-35.65, 5.90-35.25, 7.00-42. 00, 6. 20-37. 15 and 6. 05-36.4 mg·L^-1 （r =0. 999 4,0. 999 2,0. 999 4, 0. 999 2,0. 999 2,0. 994 5,0. 999 0,0. 999 2 and 0. 998 4）. Their recoveries were 102. 1% ,102. 3% ,100. 6% ,103.3% ,104. 1% , 103.2% ,96. 42% ,102. 5% and 101.5% , with RSD being 1.5% ,0. 96% ,1.9% ,1.3% ,1.7% ,2. 5% ,0. 62% ,2. 9% and 1.3%. The content of triterpenes contained in G. lucidum samples from 31 different areas and under different cultivation conditions. Conclusion： The method is so feasible and highly reproducible that it can be used for quantitatie determination of the content of triterpenoid acid contained in G. lucidum.