反相高效液相色谱法测定巴豆油中游离型和结合型亚油酸的含量

Determination of free and bound type linoleic acid in crotonis oil by RP-HPLC

目的建立巴豆油中游离型和结合型亚油酸的含量测定方法,为巴豆油的质量评价提供理论依据。方法采用RP-HPLC法,色谱条件:Kromasil 100-5C18(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-0.1%甲酸(80:20),等度洗脱;流速:1.0 mL min-1;柱温:25℃;检测波长:210 nm。结果巴豆油中亚油酸在0.04～0.68 mg mL-1与峰面积呈良好的线性关系(Y=6.000×106X+6.916×104,r=0.999 7,n=7),平均回收率为98.0%(游离型)和98.5%(结合型)(n=6)。结论该方法简单、准确、重复性好,可为巴豆药材的质量控制提供参考。

Objective To establish an RP-HPLC method for the determination of linoleic acid in crotonis oil,and to offer evidence for quality evaluation of fructus crotonis.Methods RP-HPLC method was used.The separation condition was as follows： Kromasil 100-5C18 chromatographic column（250 mm×4.6 mm,5 μm）,elution was used with the mobile phase of acetonetrile-0.1% formic acid（80 ： 20）,the flow rate was 1.0 mL ？ min-1,the column temperature was 25 ℃,and detection wavelength was 210 nm.Results The linear range of linoleic acid in crotonis oil was 0.04-0.68 mg ？ mL-1.The correlation coefficient of calibration curves was 0.999 7.The average recovery was 98.0%（free type） and 98.5%（bound）（n=6）.Conclusion The established method is simple,accurate,and repeatable,which will supply evidence for the quality evaluation of fructus crotonis.