摘要
目的探讨将钙视网膜蛋白(CR)用于诊断先天性无神经节细胞症(HD)的可行性。方法采用免疫组织化学法检测CR在50例HD(HD扩张段组、HD痉挛段组)和10例对照组结肠壁的表达,并与常规HE染色进行对比分析。用Image-Pro-Plus图文分析系统定量分析染色结果。结果 HD痉挛段组与对照组及HD扩张段组黏膜下层、肌层间CR表达的平均光密度值(MOD)差异均有统计学意义(P<0.01);HE染色扩张段肠壁神经丛中可见神经节细胞,痉挛段神经节细胞缺失,CR免疫组织化学染色与HE染色诊断结果一致;新生儿与年长儿HD痉挛段黏膜下层CR表达差异无统计学意义(P=0.816)。结论 CR免疫组织化学染色用于新生儿HD的诊断与传统方法比较具有明显优越性。
Objective To explore the feasibility of calretinin（CR） in the diagnosis of hirschsprung′s disease（HD）.Methods Colons from 50 children with HD（HD spastic segments group and ecstatic segments group） and 10 controls were stained for CR by immunohistochemistry,and were compared with conventional HE staining,with Image-Pro-Plus analysis system for quantitative analysis of staining.Results There was a significant difference in the expression of calretinin mean optical density between HD spastic segments group and ecstatic segments group,normal control group（P0.01）.Ganglion cells could be seen in ecstatic segments in HE stained section of intestinal nerve plexus,but lost in spastic segment,CR results of immunohistochemistry were consistent with HE staining results.Newborns and older children HD spastic segment submucosa had no significant difference in the expression of CR（P=0.816）.Conclusion CR immunohistochemical staining for the diagnosis of neonatal HD has obvious advantages compared with traditional methods.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第33期3468-3470,F0002,共4页
Chongqing medicine
基金
国家自然科学基金面上项目(30973136)
