碳源对微生物硝酸盐异化还原成铵过程的影响

Effect of Carbon on Dissimilatory Nitrate Reduction to Ammonium Process

微生物通过异化性硝酸盐还原成铵(DNRA)途径,硝态氮转化为仍可生物再利用的铵盐。以琥珀酸钠、柠檬酸钠、酒石酸钾钠为碳源,研究碳源的差异对有氧条件下微生物通过DNRA途径产铵的影响。结果显示,以琥珀酸钠和柠檬酸钠为碳源,初始浓度为20 mmol/L是较佳的实验条件,此时C/N约为1.5～2.0,NH4+-N质量浓度30.0～45.0 mg/L,最高产铵率分别为29.9%和27.0%;以酒石酸钾钠为碳源则在初始浓度为30 mmol/L,C/N约为2.0,NH4+-N质量浓度为40.0～45.0 mg/L时,最高产铵率为30.7%。反硝化和DNRA过程是同时存在的,培养液中NO3--N浓度的下降伴随着中间产物NO2--N的积累和NH4+-N浓度的升高。

Nitrate nitrogen can be transformed to biological reused ammonium in the way of Dissimilatory Nitrate Reduction to Ammonium（DNRA） by animalcule.These three different carbon sources that sodium succinate,sodium potassium tartrate and trisodium citrate affecting DNRA under aerobic environment have been studied in this experiment.Results show that the better initial concentration of sodium succinate and trisodium citrate should be 20 mmol/L（C/N 1.5-2.0）,on this condition,ammonium concentration rang among 30.0-45.0 mg/L and the rates of ammonium production respectively are 29.9% and 27.0%;but the better initial concentration of sodium potassium tartrate should be 30 mmol/L（C/N 2.0）,ammonium concentration changes between 40.0-45.0 mg/L and the rates of ammonium production is 30.7%.Denitrification and DNRA are coexisted and nitrate concentration decreases always accompanied with the increase of nitrite and ammonium concentration.