响应面法优化微波提取啤酒花多酚工艺研究

Optimization of Microwave Extraction Process for Hop Polyphenol by Using Response Surface Methodology

[目的]微波辅助提取法提取啤酒花多酚,为酒花浸膏的工业化生产提供科学依据。[方法]以多酚含量为响应值,在单因素实验的基础上,筛选出乙醇浓度、微波功率、微波时间三种对提取影响较大的因素,响应面法进行优化,并验证优化方案。[结果]确定酒花多酚最佳提取条件为:乙醇浓度56%,微波功率720 W,微波时间81 s。[结论]在此条件下,提取多酚含量达到81.358 mg/g。

[ Objective]The purpose of this study was to use microwave auxiliary extraction to extract hop polyphenol in order to provide the scientific basis for the industrial production of hop concrete. [ Method ] By means of single factor experiment, three main factors ： the ethanol concentration,power of microwave and the microwave time were screened at fist. And these factors were optimized by using response surface methodology. [ Result ] Results of response surface analysis showed that the optimum conditions were as follows： ethanol concentration 56%, the power of microwave 720 W, the microwave time 81 s . [ Conclusion] Under this condition, polphenol of hop polyphends to 81. 358 mg/g.