睡菜的离体培养与植株再生研究

Study on in vitro Culture and Plant Regeneration of Menyanthes trifoliata

以不带节的睡菜茎段为外植体,研究其离体再生体系。结果表明:将茎段接种到MS+6-BA 1.0mg/L+IBA 0.5mg/L培养基上10d可以诱导产生愈伤组织,然后转接至MS+6-BA 2.5mg/L+NAA 0.2mg/L+KT 0.05mg/L培养基上15d可使愈伤组织分化为幼苗;将带节的睡菜茎段接种到MS+IBA 0.1～0.2mg/L培养基上7d可使其腋芽萌发为幼苗;将幼苗接种到1/2MS+IBA 0.5mg/L培养基上3～5d可生根,实现植株再生。

Taking the stems without axillary bud of Menyanthes trifbliate explants,its in vitro culture and regeneration were studied. The results showed that putting the stern on the medium MS＋6-BA 1.0 mg/L＋IBA 0. 5 mg/L for 10 d could produce callus,then put them on MS＋6-BA 2. 5 rng/L＋NAA 0. 2 mg/L＋KT 0. 05 rng/L for 15 days,it could differentiate into seedlings without root;taking the sterns with bud on MS＋IBA 0. 1-0. 2 mg/L for 7 days,axillary bud could germinate and then be seeding without root.Put the seedling on 1/2MS＋IBA 0. 5 rng/L for 3-5 d,it could rooting.