摘要
目的对大鼠胰岛进行分离和纯化。方法胶原酶V内消化法分离胰岛,在通过Histopaque分离液1119、1083、1077配合使用HBSS缓冲液对胰岛进行纯化。结果该方法能够得到充足的胰岛,每只大鼠能够获取(758±55)个胰岛,纯度大于95%,并且与Ficoll法相比操作更加简单。结论胶原酶V内消化联合Histopaque法分离和纯化大鼠胰岛是一种简单有效的方法。
Purpose Isolation and purification of rat islets. Method Retrograde perfusion of collagenase V to pancreas in order to isolate islets, and purification of islets were performed using histopaque 1119, 1083, 1077 and HBSS. Result Enough islets were harvested （758 ±55） islets with the purity greater than 95%. This method is relatively easier and more practical compared with the ficoll method. Conclusion Isolation and purification of rat islets by using collagenase V and histopaque is a simple and effective method.
出处
《实验动物科学》
2012年第5期19-23,共5页
Laboratory Animal Science
基金
教育部博士点基金:利用基因转染和时空特异性基因打靶技术体外提高胰岛可控性增殖能力(No.20090001110096)
