鹌鹑血清中脂类代谢相关生化指标正常参考值的测定

The Determination of Normal Levels of Lipid Metabolism indices in Serum of Quail

目的建立鹌鹑血清中脂类代谢相关生化指标的正常参考值。方法饲养40只鹌鹑(5周龄,体质量150～200 g,雌雄各半),动态观察其血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的含量变化,其中TG采用终点法/GPO-PAP Method检测,TC采用终点法检测,HDL和LDL采用直接测定法检测。结果雄性和雌性鹌鹑血清中TG、TC、HDL和LDL值存在差异,且差异有统计学意义;不同取血时间,雄性鹌鹑血清中脂类代谢指标比较稳定,确定其参考值范围,TG为1.15～1.55 mmol/L、TC为4.18～5.30 mmol/L、HDL为2.38～3.90 mmol/L、LDL为0.63～2.07 mmol/L。结论初步建立了雄性鹌鹑血清中脂类代谢相关生化指标的正常参考值。

Objective To establish the reference value of lipid metabolism indicators in serum of quail. Method To breed twenty male quails and twenty female quails with the weigh between 150 g and 200 g, which were five weeks old, in compliance with conventional, and then at different time to detect lipid metabolism indicators： detecting the triglyceride （TG） using the endpoint/GPO-PAP method, detecting the total cholesterol （TC） using the endpoint method, detecting the high density lipoprotein （HDL） and the low density lipoprotein （LDL） using the direct assay method. Result Compared with the female quail, the male quail had different values of TG, TC, HDL and LDL in serum, and the difference was statistically significant; the value of TG, TC, HDL and LDL in male quail serum was stable at different detection intervals, the reference range was determined initially and it is as follows： TG 1.15 - 1.55 mmol/L, TC 4. 18 - 5.30 mmol/L, HDL 2.38 - 3.90 mmol/L and LDL 0.63 - 2.07 mmol/L. Conclusion （1）The study established the reference range of lipid metabolism indicators in male quail serum. （2）The result indicated that the female quail in laying period as animals for lipid metabolism research is not appropriate.