灵芝多糖联合低剂量顺铂抑瘤作用及对荷瘤鼠免疫功能的影响

Ganoderma lucidum polysaccharides combined with low-dose cisplatin inhibits tumor growth and enhances the immune function of tumor-bearing mice

目的研究灵芝多糖与顺铂联合用药对荷瘤小鼠的肿瘤生长情况及其免疫功能的影响。方法Balb／c小鼠腋下接种小鼠肺癌细胞株Lewis5×10^6个细胞，待肿瘤至100min^3分组给药。分为对照组、灵芝多糖组（1次／2d，40mg／kg，灌胃）、顺铂组（1次／2d，2mg／kg，腹腔注射）和联合用药组（等剂量的顺铂和灵芝多糖）。每3天测量一次肿瘤的体积，30d后解剖，检测荷瘤小鼠的肿瘤大小、脾脏的变化，脾脏和外周血中免疫细胞的变化以及相关免疫细胞的功能。结果灵芝多糖可有效增加小剂量顺铂的抗肿瘤效果，提高荷瘤小鼠的存活率，使小鼠外周血中Th细胞向Thl转化，增加细胞免疫功能，并增加骨髓来源DC细胞表面CDllc分子的表达（与对照组比较灵芝多糖组从1．06％升至4．59％，与顺铂比较，联合用药组从2．8％升至7．21％），提高抗原提呈能力。结论灵芝多糖可以提高荷瘤小鼠的免疫功能，与小剂量顺铂联合用药后，具有协同抗肿瘤生长作用，且可提高小鼠存活率。

Objective To study the effects on inhibit tumor growth and enhance immune fimction of Ganoderma lucidum polysaccharide（GLP）combined with low-dose cisplatin in tumor-bearing mice. Methods 2 × 10^6 Lewis cells in 0.1 mL PBS were injected subcutaneously into the flanks of healthy female BALB/c nude mice （5-6 weeks old）. When the tumor volume reached 100 mm3, mice were randomly assigned to 4 groups, control group （Oral gavage of 0.5 ml PBS and intraperitoneal injection of 0.5 ml）, GLP group （gavage once every two days, 40 mg/kg GLP in 0.5 ml）, cisplatin group （intraperitoneal injection once every two days, 2 mg/kg cisplatin in 0.5 ml） and the combination group （such as the above dose of cisplatin and GLP）. After the treatment regimen was complete, the mice were sacrificed, and the tumors were removed, photographed, weighed, and prepared for histological analysis. The immune cells in spleen and peripheral blood were analyzed by flow cytometry. Results GLP can effectively increase anti-tumor effect of the small dose cisplatin, improve the survival of tumor-bearing mice, promote Th ceils convert to Thl in peripheral blood, increased the expression of CDllc＋ on DC cell surface （GLP group 4.59% VS the control group 1.06%, the combined group 7.21% VS the cisplatin group 2.8%）. Conclusion GLP can improve the immune function of tumor-bearing mice, had synergistic anti-tumor growth with low dose cisplatin, moreover, reduce renal toxic effects of cisplatin.