摘要
利用自组装单分子层(SAMs)/普鲁士蓝(PB)/壳聚糖(Chit)界面固定葡萄糖氧化酶(GOD),构建酶生物传感器,对传感器在极性非质子有机溶剂如乙腈(AN)、二甲基亚砜(DMSO)和N,N-二甲基甲酰胺(DMF)存在条件下的电化学特性进行了研究,考察了有机溶剂体积分数对传感器响应的影响。结果表明,传感器在50%(体积分数)AN中的电流响应可达到磷酸缓冲溶液(PBS)中的73%。传感器响应速度快、重现性好、抗干扰能力强,展现了高的灵敏度(57.3mA.M.cm-2)。该传感器用于血清标本中葡萄糖的检测,测定结果与市售葡萄糖酶光度测定试剂盒测定结果相吻合。
A biosensor was fabricated with glucose oxidase （GOD） immobilized on self-assembled monolayers （SAMs）/Prus sian blue （PB）/chitosan （Chit）. The electrochemical characteristics of the biosensor were studied in the presence of dipolar aprotic organic solvents such as acetonitrile （AN）, dimethyl sulfoxide （DMSO） and N, N-dimethyl formamide （DMF）. The effect of volume percentage of organic solvents on biosensor response was investigated. It is observed that the response current of the biosensor in 50% AN reaches 73% of that in phosphate buffer solution （PBS）. The amperometric signals are fast, re producible and exhibiting high sensitivity （57. 3 mA · M · cm-2）. The biosensor was used to determine glucose in serum speci mens, and the results are consistent with those obtained with the commercially available glucose enzyme photometric kit.
出处
《河北科技大学学报》
CAS
2012年第5期391-396,共6页
Journal of Hebei University of Science and Technology
基金
河北省自然科学基金资助项目(B2010000844)
