PC-3悬浮成球细胞中ABCG2表达及其对化学治疗耐药性的实验研究

Experimental studies on the ABCG2 expression and chemoresistance of suspension sphere-forming PC-3 cells

目的:探讨人雄激素非依赖性前列腺癌PC-3悬浮成球细胞中ATP结合盒膜转运蛋白G超家族成员2(ABCG2)的表达及其对化学治疗的耐药性。方法:体外无血清悬浮培养PC-3细胞,逆转录定量-PCR检测PC-3悬浮成球细胞中ABCG2 mRNA的表达,采用MTT法检测PC-3悬浮成球细胞对顺铂的耐药性,计算半数抑制浓度(IC50),并与PC-3贴壁细胞作对比。结果:PC-3悬浮成球细胞可以在无血清培养条件下生存并形成悬浮细胞球,PC-3悬浮成球细胞中ABCG2 mRNA的相对表达水平是PC-3贴壁细胞的33.98倍(P<0.05),其IC50是PC-3贴壁细胞的4.26倍(P<0.05)。结论:人PC-3悬浮成球细胞中ABCG2表达水平较高,该类细胞对化学治疗具有较强耐药性。

Objective： To explore the ABCG2 expression and the chemoresistance of human prostate cancer sphere-forming PC-3 cells. Methods： PC-3 ceils were suspension cultured in vitro with a serum-free medium （SFM） . The expression of ABCG2 mRNA in sphere-forming PC-3 cells was detected with Real-time quantitative PCR （qRT-PCR） . The sensitivity of sphere-forming PC-3 cells to cisplatin was determined with MTr. Results： The PC-3 sphere-forming cells could survive in SFM and form floating cell spheres. The relative expression quantity of ABCG2 mRNA in the sphere-forming cells was 33.98-fold higher than that of the PC-3 adherent cells （ P 〈 0.05 ）. The IC50 of the sphere-forming cells was 4. 26-fold higher than that of the PC-3 adherent cells （P 〈0. 05）. Conclusion： The ABCG2 expression of humanprostate cancer sphere-forming PC-3 cells is high, and the chemoresistance of PC-3 sphere-forming cells is very strong.