超微粉碎预处理沙柳原料酶解条件优化

Optimization of ultrafine jet mill pretreatment conditions for enzymatic hydrolysis of Salix psammophila

研究了超微粉碎预处理技术在沙柳原料酶水解中的应用。沙柳原料经过超微粉碎处理后,通过正交实验设计优化原料的稀酸高温预处理条件,得到最佳的预处理参数为:原料粒度(15μm)、固液比1:12、温度160℃、H2SO40.5%、时间20min;经过预处理的沙柳原料通过混料实验设计法对纤维素酶的复配条件进行优化,得到最佳的纤维素酶系比例为:滤纸酶活(FPU):CMC酶活(IU):β-葡萄糖苷酶(IU):木聚糖酶(IU)=1.52:5.35:29.55:1,该酶系比例的纤维素酶比使用单一商品纤维素酶水解效率提高了28%以上。

The application of ultrafme jet mill pretreatment of Salix psammophila in its enzymatic hydrolysis was explored. Orthogonal experimental design was used to optimize the pretreatment conditions of milled Salix psammophila. The optimal pretrcatment parameters were： particle size （15μm）, solid-liquid ratio 1：12, 160%, H2SO4 0.5%, and mixture time 20min. Mixture design was applied for the optimization of cellulase complex formula for enzymatic hydrolysis ofpretreated Salix psammophila. The optimal composition of ceUulase complex was as follows：filter paper activity （FPU）：CMC activity （IU）：β-glucosidase activity （IU）：xylanase activity（1U） =1.52：5.35：29.55：1. Using cellulase complex, the hydrolysis rate of Salix psammopbila was increased by 28% than by using individual cellulase.