猫棒束孢真菌液体发酵工艺研究

Optimization of submerged fermentation conditions of Isaria felina

经过对培养基、装量、温度、发酵时间等因素的考察，确定了猫棒束孢最佳发酵工艺：培养基为蛋白胨0．6％，酵母膏1．2％，蔗糖2．4％，硫酸镁0．05％，磷酸二氢钾0．1％，用饱和碳酸氢钠溶液调整pH值为6．5～7．O；培养基装量为500mL;发酵温度为26．5℃；发酵时间为48h。

Effect of culture medium, culture medium volume, culture temperature and culture time on mycelia dry weight of Isaria felina was studied. The optimum medium was as followed： peptone 0.6%, yeast extract 1.2%, sucrose 2.4%, MgSO4 0.05%, KH2PO4 0.1%, pH 6.5-7.0 adjusted by satu- rated solution of NaHCO3, medium volume 500ml/3000ml shaking flask, culture temperature 26.5℃ and culture time 48h.