鸡骨草防治大鼠脂肪肝的实验研究

Experimental Research on Prevention and Treatment of Fatty Liver Rats with Abrus cantoniensis

目的:探讨鸡骨草对脂肪肝大鼠血脂代谢及其肝脏病理、肝窦内皮细胞窗孔形态学的影响。方法:将SD大鼠随机分为正常对照组、模型组、辛伐他汀组(7.2 mg/kg)、绞股蓝组(16.2 mg/kg)及鸡骨草高、中、低剂量组(40、20、10 g/kg),高脂饲料喂养3 w建立脂肪肝模型后,各药物组给予药物灌胃3 w并继续给予高脂饲料。第43天采血测转氨酶和血脂四项,并取肝组织作病理切片,光镜下观察肝组织,或在体灌注后取肝组织固定,经处理后在扫描电镜下观察肝窦窗孔变化。结果:鸡骨草高剂量能降低高脂模型大鼠血清中AST、ALT、TC、TG、LDL-C并升高HDL-C含量,在光镜和扫描电镜下亦观察到其肝组织形态以及LSEC窗孔恢复趋向正常,中剂量作用稍弱,低剂量效果不明显。结论:鸡骨草可调节高脂模型大鼠血脂水平,并改善肝脏组织的病理变化,具有降脂、保肝作用,能在一定程度上防治脂肪肝。

Objective ：To investigate the effects of Abrus cantoniensis （AC） on blood lipid metabolism, pathomorphological change of the liver and fenestrae of liver sinus endothelial cell（LSEC） in fatty liver disease rats. Methods：SD rats were divided into 7 groups： blank control group, fatty liver model group, simvastatin group （ 7. 2 mg/kg）, Gynostemma pentaphyllum group （ 16. 2 mg/kg）, high dose （40 g/kg） ,middle dose （20 g/kg） and low dose （ 10 g/kg） of AC groups. All rats except blank control group were fed with high fat diet for the first 3 weeks, then treated with different conditions as previously mentioned for the next 3 weeks while keep on feeding with high fat diet. At the 45rd day,the abdominal aortic blood was collected for measuring the serum concentration of AST,ALT,TC, TG, HDL-C, LDL-C, and liver tissues were taken to make pathological sections for observation by optical microscope or were prepared for scanning electronic microscope. Results ： The levels of AST, ALT, TC, TG, LDL-C were obviously decreased while HDL-C were in- creased in fatty liver rats by AC high dose. Meanwhile the cell morphology of liver tissues and the fenestraes of LSEC were improved as well. Conclusion：AC can ameliorate the levels of blood lipid in fatty liver rats and improve the pathological change of liver tissues. To some extent AC has the function of prevention and treatment of fatty liver.