中性蛋白酶法提取富硒羊肚菌菌丝体多糖的研究

Study on the Extraction of Mycelium Polysaccharides from Selenium-rich Morchella esculenta with Neutral Proteinase Method

目的:研究中性蛋白酶法提取富硒羊肚菌菌丝体多糖的条件,以提高菌丝体多糖的得率。方法:在单因素试验的基础上,进行正交试验设计,考察加酶量、酶解时间、酶解温度、料液比等因素对多糖提取率的影响。结果:确定了中性蛋白酶法提取富硒羊肚菌菌丝体多糖的最佳工艺:加酶量为1.5%,提取时间为2 h,酶解温度为40℃,料液比为1∶15,在此条件下,提取率可达11.26%。结论:本工艺简单、稳定、可行,适用于富硒羊肚菌菌丝体多糖的提取。

Objective：To study the extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase for increasing of yield. Methods ： On the basis of single factor tests, orthogonal experiment design was applied to ana- lyze the influence of factors including enzyme dosage, enzymolysis time, enzymolysis temperature and material/liquid ratio on the extrac- tion rate of the polysaccharides. Results ： The optimal extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta was as follows： enzyme dosage was 1. 5 %, enzymolysis time was 2 h, enzymolysis temperature was 40 ℃, and material/liquid ratio was 1： 15. The extraction rate of polysaccharide was 11.26 % under the extraction conditions. Conclusion： The process is simple, stable and practicable, and can be used for the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta.