两种猪肺炎支原体原位杂交检测方法的应用

Comparative study on two in situ hybridization methods of Mycoplasma hyopneumoniae

为了比较研究3种猪肺炎支原体(Mhp)的检测方法,利用已建立的显色原位杂交(CISH)和量子点荧光原位杂交(QD-FISH),并结合PCR方法,检测人工感染Mhp的试验猪、自然感染Mhp的试验猪和疑似感染Mhp的临床样品。结果显示,3种检测方法对人工感染组和自然感染组的检测率均为100%;对临床样品,PCR的检测率为90%,CISH的检测率为70%,QD-FISH的检测率为75%。结果表明,用于检测Mhp感染,PCR是最佳检测方法,但CISH和QD-FISH在Mhp检测和致病机理研究方面具有一定的应用价值。

The present study was designed to compare three methods for detecting Mycoplasrna hyopneumoniae （Mhp）. PCR, chromogenic in situ hybridization（CISH） and Quantum dots fluorescent in situ hybridization（QD-FISH） ,were used to detect lung tissue samples collected from experimentally infected pigs, naturally infected pigs and suspected infected pigs. The results showed that the CISH and QD-FISH experiment results of artificial infection group and natural infection group were consistent with the results of PCR method. The detection rate of PCR, CISH and QD-FISH for clinical samples were 90%, 70% and 75 %, respectively. Thus, it could be concluded that CISH and QD-FISH had a certain value in the detection and pathogenesis research of Mhp,while PCR was the best method for detecting Mhp.