肝移植术后早期白细胞分类计数与T淋巴细胞亚群计数的相关性研究

Correlation between differential blood count and T-lymphocyte subset count in the early period after liver transplantation

目的探讨肝移植术后早期白细胞分类计数与T淋巴细胞亚群计数的相关性。方法收集84例行经典原位肝移植术受者术后第7天在不应用糖皮质激素情况下的血样本,分析T淋巴细胞亚群计数与白细胞计数、中性粒细胞计数、淋巴细胞计数及单核细胞计数的相关性。结果 Pearson分析显示,CD4+和CD8+T淋巴细胞计数与白细胞计数、中性粒细胞计数及单核细胞计数间均不相关(P值均>0.05),与淋巴细胞计数间均呈正相关(R值分别为0.778、0.651,P值均<0.001)。Spearman分析显示,CD4+和CD8+T淋巴细胞计数与白细胞计数、中性粒细胞计数及单核细胞计数间均不相关(P值均>0.05),与淋巴细胞计数间均呈正相关(R值分别为0.748、0.702,P值均<0.001)。非感染组的淋巴细胞计数、CD4+T淋巴细胞计数、CD8+T淋巴细胞计数均显著高于感染组(P值均<0.05)。结论肝移植术后早期淋巴细胞计数与CD4+T淋巴细胞计数具有较好的正相关性,可间接反应CD4+T淋巴细胞计数水平。

Objective To explore the correlation between differential blood count and T-lymphocyte subset count so as to provide a method to quickly identify cellular immune function in the early period after liver transplantation.Methods Blood samples were taken from 84 recipients（without application of glucocorticoid） on day 7 after orthotopic liver transplantation（OLT）.The correlation between T-lymphocyte subset count and differential blood count including leucocyte count,granulocyte count and monocyte count was analyzed.Results Pearson analysis showed that the counts of CD4^＋ and CD8^＋ T lymphocytes were not correlated with the count of leucocyte,granulocyte and monocyte（all P0.05）,but were positively related to lymphocyte count（R=0.778,0.651;both P0.001）.Spearman analysis also showed that the counts of CD4^＋ and CD8^＋ T lymphocytes were not correlated with the count of leucocyte,granulocyte or monocyte（all P0.05）,and were positively related to lymphocyte count（R=0.748,0.702;both P0.001）.The counts of lymphocyte,CD4^＋ T lymphocyte and CD8^＋ T lymphocyte in the patients without infection were significantly higher than those in infected patients（all P0.05）.Conclusion The count of lymphocyte is positively correlated with the count of CD4^＋ T lymphocyte in the early period after liver transplantion.（Shanghai Med J,2012,35：881-883）