枯草芽孢杆菌凝乳酶高产菌株的微波诱变

The Breeding of the Rennet High-producing Strain from Bacillus subtilis and Studies on Conditions for Microwave Irradiation

以1株产凝乳酶枯草芽孢杆菌菌株为对象,对其进行微波诱变处理,考察诱变时间、诱变频率对菌株诱变效果影响,得到最佳诱变条件为:诱变时间为4 min,诱变功率为450 W;获得1株高凝乳酶活菌株(菌株编号为BsB1.1),其遗传稳定性良好,凝乳酶活达到516.12 U/g,比出发菌株提高了29.0%。

was obtained. original strain. power was 450 After the strain of Bacillus subtilis was treated with Microwave Irradiation, a high rennet activity strain Rennet activity produced by BsB1.1 reached 516.12U/g, which was increased by 29% compared with The tests of microwave irradiation conditions showed that the optimal time was 4min and the optimal W.