肝硬化患者中白细胞介素17A水平及临床意义

Interleukin-17A level in patients with liver cirrhosis and its clinical significances

目的:研究肝硬化患者中白细胞介素(Interleukin,IL)-17A的表达情况与其定位,并研究外周血和组织中IL-17A的含量与肝硬化发展的关系。方法:收集肝硬化患者外周血和肝组织,通过血清酶联免疫吸附法和免疫组织化学法,观察IL-17A在肝硬化患者血清、肝脏组织中的表达量,在肝硬化组织中的定位及其与肝硬化程度之间的关系。结果:肝硬化患者血清中的IL-17A水平明显高于正常对照者;肝硬化组织中,IL-17A主要表达在肝星状细胞、浸润的中性粒细胞和淋巴细胞,以及胆管上皮细胞中,新鲜肝组织中IL-17A的含量与Child-Pugh分级之间存在正相关关系。结论:IL-17A在肝硬化过程中起着重要的作用,且可一定程度反映肝硬化的进展。

Objective：To observe the expression and location of IL-17A in the liver fibrosis and evaluate the clinical significances of IL-17A. Methods：The peripheral blood and liver tissue was collected from the patients with liver fibrosis. The serum IL-17A level was assayed by ELISA, and the expression and location of IL-17A in the liver tissue was detected by immunohistochemistry. Results： Serum IL-17A level was significantly higher in cirrhotic patients than in control. IL-17A was mainly expressed in hepatic stellate cells,infiltrative neutrophil and lymphocyte,and bile duets epithelium in the liver fibrosis tissue. The 1L-17A expression in liver was correlated with Child-Pugh grades. Conclusion： IL-17A is critical in liver fibrosis, and the expression level of IL-17A may indicated the severity of liver fibrosis.