期刊文献+

免疫磁珠法在分离纯化外周血CD4^+和CD8^+ T淋巴细胞亚群中的应用 被引量:3

APPLICATION OF MAGNETIC ACTIVARED CELL SORTING FOR SEPARATION AND PURIFICATION OF CD4^+ T CELL AND CD8^+ T CELL SUBPOPULATIONS OF PERIPHERAL BLOOD
下载PDF
导出
摘要 目的:探讨免疫磁珠法(MiniMACS)在分离纯化外周血CD4+和CD8+T淋巴细胞亚群中的应用。方法:应用密度梯度离心法分离外周血单个核细胞(Peripheral blood mononucleate cells,PBMC),采用MiniMACS分别分离和纯化39例标本外周血PBMC中的CD4+T淋巴细胞和CD8+T淋巴细胞,并经流式细胞仪分析细胞纯度和台盼蓝染色的方法对细胞活力进行评估。结果:MiniMACS分离外周血CD4+T淋巴细胞前、后细胞纯度分别为(37.38±5.74)%、(97.75±1.03)%(P<0.001),CD8+T淋巴细胞分离纯化前后细胞纯度分别为(20.11±6.83)%、(96.85±1.86)%(P<0.001);外周血分离前PBMC细胞活力为(97.66±2.73)%,纯化为CD4+T淋巴细胞和CD8+T淋巴细胞后细胞活力分别为(97.44±3.08)%、(98.05±2.92)%(P>0.05)。结论:MiniMACS可以高度富集CD4+T淋巴细胞和CD8+T淋巴细胞且不改变细胞的活力。 Objective: To evaluate the separation and purification of T lymphocyte subsets by immunomagnetic beads. Methods: Peripheral blood mononucleate cells (PBMC) were isolated using lymphocytes separation medium and desity gradient centrifugal action. CD4+T and CD8+T lymphoeytes were isolated and purified from PBMC by magnetic activated cell sorting (MiniMACS). The purity and activity of CD4+T and CD8+T cells were measured using flow eytometry, trypan-blue dye exclusion test, etc. Results: The results showed that the percentage of CD4+ T cells before and after the purification was (37.38 ± 5.74) and (97.75 ± 1.03)% ( P 〈0. 001) ; the pereentage of CD8+T cells before and after the purification was (20.11 ± 6.83) % and (96.85 ± 1.86) % ( P 〈0. 001), resprectively; and the cell ability was not affected by purification procedure ( P 〈0.05). Conclusion: MiniMACS can sort out effectively CD4+T and CD8+T cell subpopulations of peripheral blood and does not affect the cell vitality.
出处 《广西医科大学学报》 CAS 2012年第5期679-681,共3页 Journal of Guangxi Medical University
基金 广西科学基金资助项目(桂科自0640096) 广西科学研究与技术开发计划项目(桂科自0007026)
关键词 免疫磁珠法 T淋巴细胞亚群 分离纯化 magnetic activated cell sorting subsets of T lymphocytes separation and purification
  • 相关文献

参考文献5

二级参考文献25

  • 1谭晓玉,王霞莉,王纪敏.流式细胞技术用于脑脊液细胞分析方法学研究[J].医学检验与临床,2008,20(4):17-19. 被引量:4
  • 2赵德明,黄辉,陈卓,程焕光,尹世杰.病毒性脑(膜)炎患者脑脊液淋巴细胞CD_3和CD_(20)的表达[J].临床神经病学杂志,2005,18(2):137-138. 被引量:2
  • 3尹俊华,宋水江.原发性中枢神经系统血管炎[J].国外医学(神经病学.神经外科学分册),2005,32(2):140-143. 被引量:8
  • 4张为,曾丽.CD4^+T细胞及其亚群与多发性硬化[J].中国临床康复,2006,10(4):156-158. 被引量:11
  • 5Van Acker JT, Delanghe JR, Langlois MR, et al. Automated flow cytometric analysis of cerebrospinal fluid[J]. Clin Chem, 2001, 47:556-560.
  • 6de Graaf MT, Smitt PA, Luitwieler RL,et al. Central memory CD4+ T cells dominate the normal cerebrospinal fluid[J]. Cytometry Part B (Clinical Cytometry), 2011,80B:43-50.
  • 7Min-Fang GUO, Ning JI, Cun-Gen MA. Immunologic pathogenesis of multiple sclerosis[J]. Neurosci Bull, 2008, 24(6):381-386.
  • 8Goverman J, Perchellet A, Huseby ES, et al. The role of CD8+ T cells in multiple sclerosis and its animal models[J]. Curr Drug Targets Inflamm Allergy, 2005, 4(2):239-345.
  • 9Viglietta V, Baecher Allan C, Weiner HL, et al. Loss of functional suppression by CD4+CD25+ regulatory T cells in patients with multiple sclerosis[J]. J Exp Med, 2004, 199(7):971-979.
  • 10Robertson MJ, Schacterle RS, Mackin GA, et al. Lymphocyte subset differences in patients with chronic fatigue syndrome, multiple sclerosis and major depression[J]. Clin Exp Immunol, 2005,141(2):326-332.

共引文献8

同被引文献29

引证文献3

二级引证文献24

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部