常州地区丙型肝炎病毒基因分型及临床分析

Hepatitis C Virus Genotyping in Changzhou Region and Its Clinical Analysis

目的:了解常州地区丙型肝炎病毒基因型分布、病毒复制与肝功能损害的关系。方法:对150例丙型肝炎病毒抗体检测为阳性的患者,采用荧光定量聚合酶链反应技术检测血清丙型肝炎病毒核糖核酸(HCVRNA)水平,并检测丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)等肝功能相关指标,HCV基因分型采用基因芯片法。结果:150例患者中,HCV RNA阳性患者130例(>1×103IU/ml),HCV RNA阴性患者20例(<1×103IU/ml)。经相关性检验,HCV RNA阳性患者血清ALT、AST水平与HCV RNA含量表明无显著的相关性(P>0.05),HCV RNA阳性患者血清ALT和AST指标明显高于HCV RNA阴性患者(P<0.05)。对其中54例HCV RNA阳性标本进行HCV基因分型,1型有46例(85.19%),非1型8例(14.81%)。1型与非1型相比较,两组的血清ALT和AST水平没有明显差异(P>0.05)。结论:常州地区丙型肝炎病毒感染以1型病毒为主;持续的丙型肝炎病毒复制与肝功能损害密切相关;病毒载量与肝功能检测对丙型肝炎的治疗有重要意义。

Objective To study the distribution of hepatitis C genotypes in Changzhou region,and the relationship between the viral replication and liver dysfunction.Methods Hepatitis C virus antibody（HCV-Ab） positive serum samples of 150 patients were collected and serum hepatitis C virus RNA（HCV RNA） level was detected by using FQ-PCR and alanine aminotransferase（ALT）,aspartate aminotransferase（AST） were also detected.Besides,HCV was genotyped by gene chip.Results 150 cases were divided into two groups including HCV RNA negative group（ 1 × 10 3 IU / ml）,HCV RNA positive group（ 1 × 10 3 IU / ml）.Serum ALT and AST levels in the positive group were higher than those in the negative group（P 0.05）.Virus genotyping was performed on 54 cases and there were 46 cases of type 1（85.19%） and 8 cases of non-type 1（14.81%）.Type-1 group compared with the non-type 1 group,serum levels of ALT and AST showed no real difference significantly（P 0.05）.Conclusion Hepatitis C virus in Changzhou region is mainly type 1 virus,and sustained HCV replication may cause liver dysfunction and it is important to detect HCV RNA,ALT and AST in clinical treatment.