不同质量浓度阿仑膦酸钠对大鼠颅骨修复的影响

The Effect of Different Mass Concentration of Alendronate in Hydroxyapatite-Alendronate on Rat Critical-Sized Calvarial Defect Repairment

目的探讨不同质量浓度阿仑膦酸钠(AL)对大鼠颅骨修复的影响。方法健康3月龄SD大鼠20只,每只大鼠颅骨制备四个极限全层骨缺损,缺损之间留出2～3 mm距离,分别做充填含AL 0 mg/g(A组)、1 mg/g(B组)、2 mg/g(C组)和4mg/g(D组)的纳米羟基磷灰石-阿仑膦酸钠复合材料(nHA-AL)的处理。术后第2、4、8、12周各随机处死5只大鼠,完整切取颅骨,采用大体标本观察、X线片分析及组织学观察的方法,比较各组的缺损修复情况,用SPSS 13.0对数据进行析因方差分析。结果实验发现,C组数据均优于其他各组,通过X线片分析和组织学观察发现组间差异有统计学意义(P<0.05)。结论 AL对骨组织再生有促进作用,且促进作用随比例改变而改变,适当比例的nHA-AL有望制备成为骨支架材料,应用于临床。

Objective To eveluate the effect of different concentrations of alendronate（AL） on repairing of the defects in rat′s calvaria and the relationship between the effect and the concentration of AL.Methods Twenty three-month old healthy Sprague-Dawley rats were used in this experiment.Four critical full-thickness bone defects were made on each rat′s calvaria,and 2～3 mm distance were left amang bone defects and then nanohydroxyapatite-alendronate（nHA-AL） what contains different concentrations of alendronate（group A 0 mg/g,group B 1 mg/g,group C 2 mg/g and group D 4 mg/g） was filled in the four bone defects,respectively.Conventional feeding was implemented after operation and antibiotic was used as routine.5 rats randomly selected were killed at 2 ed,4 th,8 th week and 12 th week after surgery,and their calvarias were obtained completely with low-speed motor.Bone regeneration in all groups was compared with general observation,X-ray tset and pathohistological observation.Image-proplus 6.0 was used for analyzing the residual area in each defect.SPSS 13.0 sofeware package was used for SNK and LSD study.Results Animal experiments showed that group C was better than other groups in general observation,X-ray test and pathohistological observation,and X-ray and pathohistological observation result indicated that there was significant difference among groups（P0.05）.Conclusion This result indicated that bone regeneration could be promoted significantly by AL,and the stimulative effect was changed with the concentration of AL.Further more,nHA-AL maybe a candidate of bone scaffold materials for clinical application.