高效液相色谱法测定氨苯蝶啶片的含量

Determination of the content of Triamterene tables by HPLC

目的建立高效液相色谱法测定氨苯蝶啶片的含量的方法。方法采用高效液相色谱法,色谱条件：Waters Symmetry C18（150×4.6mm,3,5μm）色谱柱;流动相为甲醇-0.02mol.L-1醋酸钠溶液（冰醋酸调节pH 4.0）（6∶4）;检测波长：360nm;流速：1.0mL.min-1。结果氨苯蝶啶在8.72~87.20μg.mL-1的浓度范围内与峰面积呈良好的线性关系（r=0.9999）,最低检出限为0.0218μg.mL-1,平均回收率分别为100.2%;RSD分别为0.60%.结论本方法专属性好,准确,灵敏,适用于氨苯蝶啶片的含量测定,结果可靠。

OBJECTIVE To establish an HPLC method for the determination of the content of Triamterene tables.METHODS HPLC analysis was carried out by using Waters Symmetry C18column（150×4.6mm,3,5μm） and a mixture of Methol and 0.02mol·L-1 sodium acetate,make up the pH 4.0with the acetic acid（60∶40） as mobile phase.The detection wavelength was 360nm and flow rate was 1.0mL.min-1.RESULTS The linear range was 8.72~87.20μg·mL-1 respectively（r=0.9999）,the limit of detection was 0.0218μg·mL-1 and the average recovery was 100.2%,（0.60%） respectively.CONCLUSION This method is simple,accurate,sensitive and reliable for determination of Triamterene tables.