大豆疫霉慢生长菌株HN-1的生物学特性及传染规律

Biological characteristics and transmission rules of the slowly-growing Phytophthora sojae isolate HN-1

观察和测定了大豆疫霉(Phytophthora sojae)慢生长菌株HN-1及其单游动孢子后代的培养性状及致病力,并将其与正常生长的其他3个菌株进行对峙培养,研究其慢生长特性传染规律,测定受感染菌株的培养特性及致病力。结果表明,大豆疫霉慢生长菌株HN-1的培养特性与正常菌株相比生长显著缓慢,菌落形态有明显差异,卵孢子产生量低,对大豆幼苗的致病力显著较弱;该菌的慢生长特性可通过无性繁殖传递至子代,并可通过对峙培养传染至其他正常生长菌株,传染后产生的受染菌株表现慢生长特性且致病力减弱。

The culture characters and pathogenicity of slowly-growing isolate HN-1 of Phytophthora sojae and its single-zoospore progeny were observed and measured, and the transmission rules and culture characters, pathogenicity of the infected cultures from HN-1 were researched through dual culture with other 3 normally-growing isolates. The results showed that the slowly-growing isolate HN-1 of Phytophthora sojae had significant difference on culture characters, growth rate and oospore production compared with normal-growing isolates. HN-1 was confirmed to be weak pathogenic isolate through inoculation in vivo. The characteristic of slowly-growing of HN-1 could be transferred to normal-growing isolates and the cultures through hyphal contact expressed slowly-growing characteristic and their pathogenicity were weakened.