石见穿多糖抑制人胃癌细胞系MGC-803细胞迁移及其机制的初步研究

Effect of the Salvia chinensis Benth.Polysaccharides on the metastasis ability of human gastric cancer cells (MGC-803 cells)

目的观察石见穿多糖对人胃癌细胞系MGC-803细胞迁移能力的影响,并探讨其作用机制。方法利用水萃取法制备石见穿多糖,通过划痕实验、Transwell实验观察不同浓度对石见穿多糖同样条件培养下胃癌MGC-803细胞迁移能力的影响,并采用Western blot方法检测不同浓度石见穿多糖对MGC-803细胞内白介素8(IL-8)蛋白表达水平的影响。结果同未加药物对照组相比,经高浓度石见穿多糖(50、100 g/L)处理后,MG-63细胞的划痕愈合能力明显减弱,穿膜细胞的数量显著减少;同时,细胞内IL-8蛋白的表达水平明显降低,并与石见穿多糖的浓度有关。结论石见穿多糖抑制了胃癌MGC-803细胞的迁移能力,其作用与抑制MGC-803细胞内的IL-8蛋白表达水平有关。

Objective To investigate the effect of Salvia chinensis Benth.Polysaccharides（SBP） on the metastasis ability of hu man gastric cancer cell line（MGC-803 cells） in vitro,and to explore the action mechanisms of SBP.Methods SBP prepared by extraction of water,the effect of the different concentration of SBP on the experimental metastasis ability of MGC803 cells was observed by scratch test and Transwell metastasis experiments.The level of IL-8 protein in MGC-803 cells were detected by Western Blotting.Results Compared with control group（no drug）,SBP（50 and 100 g/L） inhibited the MGC-803 cells wound,MG-63 cells scratches healed was significantly weakened,number of cells passing through filter membrane would decrease.The level of IL-8 protein in MG-63 cells decreased significantly after treated with SBP and correlation with the concentration of SBP.Conclusion SBP ia able to inhibit the metastasis ability of MGC-803 cells.The action mechanisms of SBP are related to the inhibition of IL-8 protein levels in MGC-803 cells.