摘要
用周宁魔芋接种,获得最佳外植体为球茎上切取的皮上芽苞组织;用不同激素配比,筛选出愈伤组织诱导最佳培养基为MS+6-BA0.5mg/L+NAA1.0mg/L,诱导率达94.9%;最佳芽分化培养基为MS+6-BA1.5mg/L+NAA0.1mg/L,分化率达95.2%;芽在MS+NAA0.3～0.5mg/L培养基上都能100%生根形成完整植株.
The optimum explants to be gotten were budllets using konjak as inoculating materials. At different dose of hormone, MS＋6-BA0.5mg/L＋NAA1.0mg/Lappeared to be optimum for callus induction, with an induction rate of 94.9%.For regeneration of plantlets from the callus, and the optimum medium was MS＋6-BA1.5mg/L＋NAA0.1mg/L, with a differentiation rate of 95.2%. All the regenerated plantlets rooted readily on MS＋NAA0.3-0.5mg/L and grew into complete plants.
出处
《宁德师范学院学报（自然科学版）》
2012年第4期355-358,共4页
Journal of Ningde Normal University(Natural Science)
基金
宁德师范学院科研资助项目
关键词
魔芋
组织培养
再生植株
lbus guripingensis
tissue culture
plant regeneration
