p27在人脑胶质瘤细胞中的表达及意义

Expression of p27 in Human Glioma and Its Clinical Significance

目的探讨p27蛋白在人脑胶质瘤中的表达和定位及其与临床病理的关系。方法采用免疫组织化学方法检测20例正常脑组织标本(对照组)、118例脑胶质瘤组织标本(胶质瘤组,按Kernohn病理分级,其中Ⅰ级30例,Ⅱ级29例,Ⅲ级31例,Ⅳ级28例)中p27蛋白的表达和定位,并分析其与临床病理的关系。结果 p27蛋白在胶质瘤组低表达率(54.24%)显著高于对照组(0.00%)(P<0.001)。胶质瘤组中Ⅰ-Ⅳ级的p27低表达率分别为6.67%、41.38%、74.19%、92.86%,p27蛋白的表达与脑胶质瘤病理分级有关(P<0.001)。p27蛋白中、高表达标本定位于细胞质的比例分别为:对照组0.00%,胶质瘤组Ⅰ级7.14%、Ⅱ级29.41%、Ⅲ级57.14%和Ⅳ级100.00%;p27蛋白表达定位于细胞质者明显集中于恶性程度较高组(P<0.001)。结论 p27蛋白的表达和定位与脑胶质瘤的临床病理密切相关,可能是一个预测脑胶质瘤预后的重要指标。

Objective To investigate the relationship of p27 expression and location to clinicopathological characteristics in human glioma.Methods The expression and location of p27 were detected by immunohistochemical method in 20 normal brain tissue specimens（control group） and 118 glioma specimens（30 cases of Kernohn grade Ⅰ glioma,29 cases of Kernohn grade Ⅱ glioma,31 cases of Kernohn grade Ⅲ glioma and 28 cases of Kernohn grade Ⅳ glioma）.The relationship between p27 expression and clinicopathological characteristics was analyzed.ResultsLow expression rate of p27 in glioma was significantly higher than that in control group（54.24% vs 0.00%,P0.001）.In addition,low expression rate of p27 was,respectively,6.67%,41.38%,74.19% and 92.86% in Kernohn grade Ⅰ,Ⅱ,Ⅲ and Ⅳ gliomata（P0.001）.The proportion of specimens with moderate and high expression of p27 was,respectively,0.00%,7.14%,29.41%,57.14% and 100.00% in control group,grade Ⅰ glioma,grade Ⅱ glioma,grade Ⅲ glioma and grade Ⅳ glioma.The expression of p27 in cytoplasm was associated with a higher degree of malignant glioma（P0.001）.Conclusion The expression and location of p27 were closely related with the clinicopathological characteristics in human glioma.Therefore,p27 may be an important indicator for predicting the prognosis of glioma.