HPLC法测定氟康唑胶囊的含量

Determination of Fluconazole capsules by HPLC

目的建立氟康唑胶囊含量测定的高效液相色谱方法（HPLC）。方法采用C18色谱柱,以甲醇-磷酸盐缓冲液（pH 7.0）（45∶55）为流动相,流速为1.0 mL/min,检测波长为261 nm,柱温为30℃。结果氟康唑在0.123 4-0.987 6 mg/mL范围内与峰面积呈良好的线性关系,r=0.999 2,平均加样回收率为98.9%。结论HPLC法准确、可靠、重复性好,可作为氟康唑胶囊的含量测定方法。

Objective Purpose Fluconazole capsule content was determinated by HPLC.Methods C18 column with methanol-phosphate buffer（pH 7.0）（45∶55） as mobile phase,The flow rate was 1.0 mL/min,detection wavelength of 261 nm,column temperature of 30 ℃.Results Fluconazole in 0.123 4-0.987 6 mg/mL within the range of peak area showed good linear relationship,r=0.999 2,average recovery was 98.9%.Conclusion The HPLC method is accurate,reliable,reproducible,which can be used as a method for the determination of the content of fluconazole capsule.