刺齿凤尾蕨抗肿瘤活性成分5F的含量测定

Content Determination of Antitumor Active Component 5F from Pteris dispar

目的:建立测定刺齿凤尾蕨中抗肿瘤成分5F含量的方法,并测定其不同部位中5F的含量。方法:采用高效液相色谱法。色谱柱为HypersilC1(8250mm×4.6mm,5μm),流动相为甲醇-1%醋酸水溶液(梯度洗脱),流速为1.0mL·min-1,检测波长为242nm,柱温为30℃。结果:5F检测浓度在6～96μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9981);低、中、高浓度对照品加入量对应的平均加样回收率分别为103.33%、100.84%、100.39%,RSD分别为5.86%、3.05%、1.02%(n均为6)。5F在刺齿凤尾蕨叶中含量最高,茎中含量其次,根中含量最低。结论:本方法稳定、快速且杂质干扰小,可用于刺齿凤尾蕨中5F的含量测定。

OBJECTIVE： To establish an method for content determination of antitumor active component 5F from Pteris dispar, and to determine the content of 5F in different parts. METHODS： HPLC method was adopted. The separation was performed on Hypersil C18（250 mm×4.6 mm,5 μm）column with mobile phase consisted of methanol-1% acetic acid （gradient elution） at the flow rate of 1.0 mL·min-1. UV detection wavelength was set at 242 nm, and the column temperature was maintained at 30 ℃. RESULTS： The linear range of 5F was 6～96 μg·mL-1（r=0.998 1）. The average recoveries were 103.33%,100.84% and 100.39%,and RSDs were 5.86%,3.05% and 1.02%（n=6）.The content of 5F in the leaves of P. dispar was the highest,followed by the steam and root. CONCLUSION： The methods is stable,rapid and less interference from impurity, and can be used for the content determination of 5F in P. dispar.