草菇DNA提取方法初探

Ways of extracting DNA from Volvariella volvacea

研究了 3种 DNA提取方法对草菇 DNA提取的影响。结果表明 ,CTAB提取法 DNA产量高 ,多糖和蛋白质含量低 ,符合分子生物学研究要求 ;氯化苄提取法 DNA产量最高 ,蛋白质含量较低 ,但多糖含量高 ,需要进一步去除 ;原生质体提取法 DNA产量极低 ,而且蛋白质含量也较高 ,不适合于草菇

Three different ways of extracting Volvariella volvacea DNA was studied The result showed that the way of using Benzyl chloride got high yield of DNA and low concentration of protein but polysaccharide was higher; The way of using CTAB got a good yield of DNA and low concentration of polysaccharide and protein It was a good way for extracting Volvariella volvacea DNA The way of using protoplast got very poor yield of DNA