茶树中提纯总RNA的研究

Studies on Isolation of General RNA from Tea Plant [Camellia sinensis(L )O Kuntze]

在克氏一步法分离总RNA的基础上 ,根据茶树叶片生化成分特点 ,做了部分修改 ,并研究了不同浓度的L -半胱氨酸和可溶性聚乙烯吡咯烷酮 (PVP)、不溶性PVP对提取的RNA纯度、回收率和完整性的影响。分光光度计检测和变性琼脂糖凝胶电泳结果证明 :使用本方法并加入 5- 10mmol LL -半胱氨酸或 4 % - 6 %可溶性PVP能提高RNA纯度和 (或 )回收率 ,研磨时加入5% - 10

Chomczynskis single step method of RNA isolation was partially modified on the basis of the features of biochemistry compositions in fresh tea leaves Influence of different concentration of L Cysteine,soluble polyvingl pyrrolidone(PVP)and insoluble PVP on isolated RNA purity,recovery rate and interity were studied The results of the spectrometeric determination and denaturing agarose gel electrophoresis showed that the purity and/or recvovery of isolated RNA can be improved by adding 5-10 mmol/L of L Cysteine or 4%-6% of soluble PVP The integrity can be increased by adding 5%-10% of insoluble PVP when crushing the leaves