固定化果胶酶抑制铜绿微囊藻生长研究

Growth Inhibition Effect of Immobilized Pectinase on Microcystis aeruginosa

为证实固定化果胶酶抑制蓝藻生长的作用,在实验室条件下,以铜绿微囊藻(Microcystis aeruginosa)为受试藻种,用共培养法观察了固定化果胶酶对藻细胞群体的作用、用电镜观察了共培养后藻细胞的损伤状况,测定了对其生理生化特征的影响.结果表明固定化果胶酶与藻共培养液第3 d明显黄化,且黄化程度与固定化果胶酶的用量和培养时间呈正相关系;电镜照片显示固定化果胶酶对藻细胞有损伤作用,轻微损伤的藻细胞出现质壁分离,表面粗糙、凸凹不平,形状不规则,严重损伤的藻细胞表面发生深度皱缩或细胞结构完全解体;随着固定化果胶酶与铜绿微囊藻共培养时间的延长,藻细胞生长量、叶绿素a含量显著降低,表明藻细胞受到胁迫和伤害,藻细胞正常的光合作用受到严重影响.丙二醛(MDA)值显示藻细胞抗氧化防御体系被破坏,细胞内发生严重膜脂过氧化.固定化果胶酶能有效抑制铜绿微囊藻细胞的生长,铜绿微囊藻生长抑制率可高达96%.

To confirm the growth inhibition effect of immobilized pectinase on algae,co-cultivation method was used to investigate the effect of immobilized pectinase on the growth of Microcystis aeruginosa.After co-cultivation,the damage status of the algae was observed through electron microscope,and the effect of immobilized pectase on the physiological and biochemical characteristics of the algae was also measured.The results showed that the algae and immobilized pectase co-cultivated solution etiolated distinctly on the third day and there was a significantly positive correlation between the extent of etiolation and the dosage as well as the treating time of the immobilized pectinase.Under electron microscope,plasmolysis was found in the slightly damaged cells,and the cell surface of these cells was rough,uneven and irregular;the severely damaged cells were collapsed or disintegrated completely.The algal yield and the chlorophyll a content decreased significantly with the increase of the treating time.The measurement of the malondiadehyde（MDA） value showed that the antioxidation system of the treated algal cells was destroyed,and their membrane lipid was severely peroxidated.The study indicated that the immobilized pectinase could efficiently inhibit the growth of M.aeruginosa,and the inhibitory rate reached up to 96%.