成都地区奶牛的血液流变性指标测定

Determination of the Parameters of Hemorrheology in Dairy Cows in Chengdu

在血瘀证及活血化瘀方药的研究中,血液流变性异常已作为判定血瘀证的重要标准之一,而奶牛的血液流变性指标尚未见有报道。作者测定了成都地区85头健康奶牛的几项血液流变性指标的正常值,数据经统计学处理。结果表明,全血粘度:高切速0.003962±0.0004023Pa·s、低切速0.005788±0.001202Pa·s,血浆粘度0.001913±0.0002904Pa·s,RBC电泳时间16.12±2.82s,RBC压积34.17±3.29％,血沉5.95±2.48mm/2h,血沉方程K值12.91±5.04;成年母牛与育成牛之间,全血低切速粘度、RBC电泳时间、血沉的均值无显著差异(P>0.05),全血高切速粘度,血沉方程K值的均值有显著差异(P<0.05),血浆粘度,RBC压积的均值有极显著差异(P<0.01);妊娠牛与未妊娠母牛之间的各项均值无显著差异(P>0.005)。本文还对测定中的一些问题进行了探讨。

The principles and methods of hemorrheolgy have been widely used to study syndrome of blood stasis and traditional Chinese prescriptions and medicines of activating blood circulation and resolving stagnation. The abnormal parameter of hemorrheology has been considered as one of the important standards for determining syndromes of blood stasis. But the report on th the normal parameters of hemorrheology in the dairy cow has not been found, therefore blood samples from 85 dairy cows (34pregnant cows, 21 empty cows and 30 replacement heifers) were obtained via jugular veins and the blood viscosity (BV), plasma yiscosity(PV), erythrocyte eletrophoretic time (EET), packed cell volume(PCV) and erythrocyte sedimentation rate (ESR)were measured. The results indicated that the average BV was 0.003962±0.0004023 pa·s (measured by B system of NXE--taper plate model viscometer at high shearing velocity of 384 see^(-1)), but when it was measured by same viscometer at a low shearing velocity of 9.6 sec^(-1), the value was 0.005788±0.001202 pa·s. the average PV (low shearing velocity) EET, PCV, ESR and K value of ESR equation were 0.001913±0.0002904 pa·s, 16.12±2.825 sec, 34.17±3.29％, 5.95±2.48mm/2h and 12.9±5.04 respectively. Statistically, there were no significant differences in BV(low shearing velocity) EET and ESR between adult cows and replacement heifers (P>0.05), whereas significant differences in BV (high cutting speed) And K value of ESR equation(P<0.05) and apparent differeences in PV and PCV(P<0.001) were observed between adult cows and replacement heifers. Between pregnant cows and empty cows there were no significant differences in all of the parameters (P>0.05) measuded in this study.