高能量膳食对中老年食蟹猴糖尿病相关基因表达的影响

Diabetes-associated Genes Expression after High Energy Diet in Middle-age and Aged Cynomolgus Monkeys

本研究选择空腹血糖值(FPG)在正常范围内(3.20 mmol/L≤FPG<5.50 mmol/L)的中老年食蟹猴(Macaca fascicularis)60只,高能量膳食诱导12个月后,将其分为正常血糖组和诱高血糖组(FPG≥5.50 mmol/L)。采用荧光定量PCR技术对2组中36个糖尿病相关基因在诱导前后外周血白细胞中的mRNA表达量进行分析。结果表明,高能量膳食诱导后,诱高血糖组FPG和甘油三酯(TG)显著高于正常血糖组(P<0.05),而胆固醇(TCHO)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)与分组无显著相关性(P>0.05)。基因表达水平上,诱高血糖组和正常血糖组均有血管紧张素转换酶基因(ACE)、肝糖原磷酸化酶基因(PYGL)、水通道蛋白基因(AQP2)等19个基因的mRNA表达量在高能量膳食诱导前后存在显著差异(P<0.05),且基因的表达模式变化一致,但诱高血糖组的mRNA表达量变化大,且三磷酸腺苷柠檬酸裂解酶(ACLY)、选择素L(SELL)、突触相关蛋白23(SNAP23)、突触融合蛋白(STX4)这4个基因的mRNA表达水平仅在诱高血糖组高能量膳食诱导前后呈差异表达(P<0.05)。

A total of 60 middle-age and aged （above 10 years） Cynomolgus Monkeys （Macaca fascicularis） with normal fasting plasma glucose （FPG） values （3.20 mmol/L ≤ FPG 〈 5.50 mmol/L） were fed by high energy diet for twelve months. After feeding, they were classified into two groups, normal FPG group and induced high FPG group （ FPG ≥5.50 mmol/L）. Diabetes-associated gene expression profiles of peripheral blood leukocytes of the two groups were analyzed using quantitative real-time PCR before and after high energy diet, respectively. The results showed that FPG and strikingly higher than normal FPG group （ P 〈 O. 05 ） , triglyceride （TG） of induced high FPG group were but there was no significant difference in high density lipoprotein （ HDL-C）, low density lipoprotein （ LDL-C）, cholesterol （TCHO） between the two feeding groups （P 〉 0. 05）. Among 36 diabetes-associated genes tested, 19 genes （including angiotensin I converting enzyme （ACE）, phosphorylase, glycogen, liver （PYGL）, aquaporin 2 （AQP2）） displayed a similar expression pattern between induced high FPG group and normal FPG group and they were significantly up-regulated or down-regulated after high energy diet feeding （P 〈 0. 05） , while expression variation of all these 19 genes were stronger in induced FPG group compared to normal FPG group. The expressions of ATP-citrate lyase （ACLY）, selectin-L （SELL）, synaptosomal-associated protein 23 （SNAP23） and syntaxin hA （STX4） were significantly different only in induced high FPG group （ P 〈 0.05）.