高羊茅草对盐碱地原油污染土壤微生物的影响

Effects of Festuca arundinacea on the microbial community in crude oil-contaminated saline-alkaline soil

采用常规土壤理化分析和Biolog-Eco微孔板鉴定系统,研究了松嫩平原盐碱土地区高羊茅草的生长对原油污染土壤pH、总盐含量和微生物群落的影响.结果表明:石油污染导致表征土壤微生物代谢活性的平均颜色变化率(AWCD)、物种多样性指数和碳源利用数增加,微生物碳源利用模式发生改变.高羊茅草对石油污染土壤有较好的修复作用,降低了土壤pH和石油烃(TPH)含量,提高了土壤含水率.高羊茅草根际土壤的AWCD和碳源利用丰富度指数明显高于裸地土壤,为其根际微生物的生长发育提供了适宜的环境.

By using the routine soil physical and chemical analysis methods and the Biolog technique, this paper studied the effects of Festuca arundinacea growth on the pH value, total salt content, and microbial community in the rhizosphere of crude oil-contaminated saline-alkaline soil in Songnen Plain of Northeast China. Crude oil contamination resulted in the increases of average well color development （AWCD）, Shannon index （H）, and carbon source utilization richness index （S）, and altered the utilization patterns of carbon sources by the microbes. F. arundinacea had greater potential to remediate crude oil-contaminated soil. This plant could decrease the soil pH and soil total petroleum hydrocarbon （TPH） content, and increase the soil water content. The AWCD and S in F. arundinacea rhizosphere soil were obviously higher than those in the soil of naked land, providing a suitable environment for the growth and development of rhizosphere soil microbes.