中晚期孕期束缚应激对子代下丘脑多巴胺能神经元发育的影响

Effects of prenatal stress on the development of dopaminergic neurons in the hypothalamus of offspring

目的研究中晚期孕期束缚应激对子代下丘脑多巴胺能神经元发育的影响。方法使用怀孕SD大鼠，实验组在孕期第15～21d进行束缚应激，对照组则不受任何干扰。子代大鼠出生的当天为出生后的第0天。在子代大鼠出生后的第1天（P1），第7天（P7）和第30天（P30），分别测子代大鼠的体重，通过免疫组织化学染色和Western Blotting，观察和比较下丘脑内合成多巴胺的关键酶酪氨酸羟化酶（TH）染色像素密度及TH蛋白水平变化。结果与正常对照组相比，中晚期孕期束缚应激子代大鼠在P1体重较低，下丘脑TH染色像素密度和TH蛋白水平上调，这种异常在P7和P30消失。结论孕期府激使子代下丘脑多巴胺神经元早期发育异常，可能是造成子代神经系统行为学异常的原因之一。

Objective To investigate the effect of prenatal stress on the development of dopaminergic neurons in hypothalamus of rat offspring. Methods Restraint stress was performed three times each day for pregnant SD rats from embryonic day 12-19 in prenatal stress group. The day that the offspring was born was defmed as the postnatal day 1. Dopamine expression in the hypothalamus of offspring was observed using the method of immunostaining on postnatal day 1, 7 and 30, respectively. Results Body weight of offspring rats in prenatal stress group significantly decreased, while the pixel intensity and level of dopamine in hypothalamus was up-regulated, compared with control group at the postnatal day 1 （P〈0.05）. This change disappeared at the postnatal day 7 and 30. Conclusions Prenatal stress had an effect on the prenatal development of dopaminergic neurons in the hypothalamus, which may induce the long-term detrimental consequences for brain function.